Build Strong Foundations- PCR, Multiplex PCR, DNA Sequencing
Master PCR/ mPCR tips; familiarize with downloadable sequence editing tools; Solve PCR failures
4.18 (64 reviews)
391
students
3.5 hours
content
May 2024
last update
$39.99
regular price
What you will learn
PCR primers - Calculations, Dilutions, Design using NCBI -BLAST tool within minutes (see the demonstration for how to........)
Learn about "other PCR flavors" for inconsistent target gene amplification by standard PCR
Confident PCR results always- 10 rule cheatsheet
Get reliable, peer reviewed primer sequences from NCBI that will work in PCR( resource included with demonstration)
When to amplify multiple exons in one PCR tube?
How to add restriction enzyme site to your DNA using primers?
Search reference database to retrieve peer-reviewed, annotated gene sequences (primer design, sequencing comparisons)
How to start the PCR failure investigation?
Visualize your DNA/PCR product by staining and confirm the PCR results
Introduce simple bioinformatic tools for visual learners
Find sequence changes in exon- by- exon sequencing
Review sequencing result from ABI sequencers for exons- Determine success of sequencing experiment(demonstration included)
View Sequence electrophoretogram with easy to use, free tools
Screenshots
Related Topics
3138304
udemy ID
5/17/2020
course created date
11/14/2020
course indexed date
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