UcanCRISPR online: CRISPR/Cas9 master class
A high-level course on CRISPR in nature and biotechnology applications. Learn the latest developments from publications.
4.00 (107 reviews)
639
students
4 hours
content
Apr 2020
last update
$49.99
regular price
What you will learn
At the conclusion of the course students should be able to design a CRISPR experiment including locating CRISPR targets in a gene of interest, determine how to produce a customized sgRNA, introduce the components in a cell, and identify altered organisms.
A comprehensive overview of CRISPR as an bacterial adaptive immune system, including the multi-step process and evolution of many types..
How to re-purpose the Cas9 RNA guided nuclease for genetic engineering.
The transformational techniques using dCas9 to target DNA, including transcriptional reprogramming, visualizing and detecting specific genes, DNA assembly, and many more...
Methods to screen for changes introduced by CRISPR.
Pros and cons for different CRISPR experimental formats, including plasmid expression, ribonucleoprotein complexes, or mRNA.
Structural knowledge about Cas9 and how this has aided design of optimized protein variants.
Ethical considerations for use of this powerful tool.
How CRISPR can store new information in a genome in genetic recording approaches.
Related Topics
2179094
udemy ID
1/27/2019
course created date
10/30/2019
course indexed date
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